RESSOURCE PERSON TO USE THE INFRASTRUCTURE
Dr. Lisbet HAGLUND
Email : firstname.lastname@example.org
Bone Engineering Labs, C10 Surgical Research
Research Institute of the McGill University Health Centre (RI-MUHC)
Montreal General Hospital, 1650 avenue Cedar
Montréal, QC H3G 1A4
Phone: 514 934-1934, ext 35380
The equipment for the infrastructure for surgical research located primarily at the Montreal General Hospital includes:
- Surgical Navigation and Live Animal Imaging: A Stryker Navigation System II like that used in human surgery is in the OR for large animal species including rabbit, dog and mini-pig. The instrument generates an accurate digital model of the surgical site to correlate the position of surgical instruments with the anatomical target.
- A Fidex Animage instrument, developed for veterinary applications, combines computed tomography, digital radiography and fluoroscopy for live imaging.
- High resolution micro CT and Histopathology: A second generation Skyscan 1172 micro CT is used for computed tomographic imaging of bone and soft tissue injected with radio-opaque materials. The instrument has an 11-megapixel X-ray camera that captures cross-sectional images up to 8,000 x 8,000 pixels/slice and software to support 2D or 3D quantitative image analysis. After imaging, specimens are processed un-decalcified for histological analysis of tissue composition or decalcified for immunochemical analysis of molecular/cell activity.
- A Leica microtome and microscopes with digital imaging capabilities.
- 3D printing and micro-mechanical testing: ZCorp PLUS 3D printers are used for custom printing porous bioceramic scaffolds loaded with growth factors like VEGF and/or polymers for surgical planning or to fill voids in surgical models of orthopaedic injury.
- An Instron FastTrack 8800 dynamic mechanical testing system is used for biomechanical evaluation of implants under physiological loading conditions.
- Cell culture and Molecular analyses: A major expansion of cell/tissue culture facilities and recruitment of Dr Haglund to RSBO in 2016 enabled expansion of the shared infrastructure into harvest of primary human cells, including intervertebral disc, mesenchymal cells (osteoblasts), tumor cells from bone metastatic cancer and mast cells from bone marrow.
- Dr Haglund’s lab also houses shared equipment for molecular analyses that includes Applied Biosystems Real time PCR, Nanoquant Plate Reader and GE Digital Image Analyzer.